Jump to content

Auramine–rhodamine stain

From Wikipedia, the free encyclopedia
Oocysts of Cryptosporidium parvum stained with the fluorescent auramine–rhodamine stain.

The auramine–rhodamine stain (AR), also known as the Truant auramine–rhodamine stain, is a histological technique used to visualize acid-fast bacilli using fluorescence microscopy, notably species in the Mycobacterium genus.[1] Acid-fast organisms display a reddish-yellow fluorescence.[2] Although the auramine–rhodamine stain is not as specific for acid-fast organisms (e.g. Mycobacterium tuberculosis or Nocardia) as the Ziehl–Neelsen stain, it is more affordable and more sensitive, therefore it is often utilized as a screening tool.

AR stain is a mixture of auramine O and rhodamine B. It is carcinogenic.

See also

[edit]

References

[edit]
  1. ^ Kommareddi S.; Abramowsky C.; Swinehart G.; Hrabak L. (1984). "Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl–Neelsen techniques in tissue diagnosis". Human Pathology. 15 (11): 1085–1089. doi:10.1016/S0046-8177(84)80253-1. PMID 6208117.
  2. ^ Abe, C. (August 2003). 結核とそれらの上達テストの実験室テストの標準化 [Standardization of laboratory tests for tuberculosis and their proficiency testing]. Kekkaku (in Japanese). 78 (8): 541–551. ISSN 0022-9776. PMID 14509226.